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A first generation bovine BAC-based physical map
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DOI：10.1051/gse:2003054.png)
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摘要
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A first generation clone- based physical map for the bovine genome was constructed combining, fluorescent double digestion fingerprinting and sequence tagged site ( STS) marker screening. The BAC clones were selected from an Inra BAC library ( 105 984 clones) and a part of the CHORI- 240 BAC library ( 26 500 clones). The contigs were anchored using the screening information for a total of 1303 markers ( 451 microsatellites, 471 genes, 127 EST, and 254 BAC ends). The final map, which consists of 6615 contigs assembled from 100 923 clones, will be a valuable tool for genomic research in ruminants, including targeted marker production, positional cloning or targeted sequencing of regions of specific interest.
Keyword:
cattle
physical map
BAC
