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A microfluidic baby machine'' for cell synchronization
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DOI：10.1039/c2lc40277g.png)
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摘要
En 中文
Common techniques used to synchronize eukaryotic cells in the cell cycle often impose metabolic stress on the cells or physically select for size rather than age. To address these deficiencies, a minimally perturbing method known as the baby machine'' was developed previously. In the technique, suspension cells are attached to a membrane, and as the cells divide, the newborn cells are eluted to produce a synchronous population of cells in the G1 phase of the cell cycle. However, the existing baby machine'' is only suitable for cells which can be chemically attached to a surface. Here, we present a microfluidic baby machine'' in which cells are held onto a surface by pressure differences rather than chemical attachment. As a result, our method can in principle be used to synchronize a variety of cell types, including cells which may have weak or unknown surface attachment chemistries. We validate our microfluidic baby machine'' by using it to produce a synchronous population of newborn L1210 mouse lymphocytic leukemia cells in G1 phase.
Keyword:
MAMMALIAN-CELLS
CYCLE SYNCHRONIZATION
SHEAR-STRESS
EXPRESSION
PROGRESSION
INHIBITORS
SELECTION
GROWTH
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被引数:
3.3W
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Rejoinder: whole-culture synchronization cannot, and does not, synchronize cells.png)
TRENDS IN BIOTECHNOLOGY
TRENDS IN BIOTECHNOLOGYIF14.9
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