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A promiscuous split intein with expanded protein engineering applications
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DOI：10.1073/pnas.1701083114.png)
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The protein trans-splicing (PTS) activity of naturally split inteins has found widespread use in chemical biology and biotechnology. However, currently used naturally split inteins suffer from an extein dependence, whereby residues surrounding the splice junction strongly affect splicing efficiency, limiting the general applicability of many PTS-based methods. To address this, we describe a mechanism-guided protein engineering approach that imbues ultrafast DnaE split inteins with minimal extein dependence. The resulting promiscuous inteins are shown to be superior reagents for protein cyclization and protein semisynthesis, with the latter illustrated through the modification of native cellular chromatin. The promiscuous inteins reported here thus improve the applicability of existing PTS methods and should enable future efforts to engineer promiscuity into other naturally split inteins.
Keyword:
chemical biology
protein engineering
intein splicing
protein semisynthesis
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9.1
论文数:
10.8W
被引数:
73.5W
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Chemical tagging and customizing of cellular chromatin states using ultrafast trans-splicing inteins.png)
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