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A qPCR-based approach targeting the microbial gene marker <i>nanA</i> of mucin-degrading <i>Akkermansia</i> in Parkinson's disease
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DOI：10.1177/1877718x261462342.png)
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Abstract
En 中文
<jats:sec>
<jats:title>Background</jats:title>
<jats:p>Parkinson's disease (PD) is a multifactorial neurodegenerative disorder increasingly linked to gut microbiota alterations. However, despite advances in fecal microbiota profiling as a non-invasive approach to disease risk assessment, its clinical utility remains limited by a lack of functionally relevant microbial biomarkers.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Objective</jats:title>
<jats:p>This cross-sectional study aimed to identify a microbial gene marker reflecting metabolic potential associated with both the presence and severity of PD.</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Methods</jats:title>
<jats:p>
Fecal samples from patients with PD (
<jats:italic toggle="yes">n</jats:italic>
= 59) and healthy controls (
<jats:italic toggle="yes">n</jats:italic>
= 65) were analyzed by 16S rRNA sequencing to characterize taxonomic profiles. Quantitative PCR (qPCR) targeted the consensus sequence of the mucin-degrading
<jats:italic toggle="yes">nanA</jats:italic>
gene (
<jats:italic toggle="yes">
nan
<jats:sub>Akk</jats:sub>
</jats:italic>
), a highly conserved within
<jats:italic toggle="yes">Akkermansia nan</jats:italic>
gene clusters. Differences in taxonomic composition and
<jats:italic toggle="yes">
nan
<jats:sub>Akk</jats:sub>
</jats:italic>
abundance were examined, and correlations with clinical severity scores evaluated in the PD group.
</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Results</jats:title>
<jats:p>
Patients with PD showed reduced abundance of short-chain fatty acid-producing taxa (
<jats:italic toggle="yes">Faecalibacterium</jats:italic>
,
<jats:italic toggle="yes">Blautia</jats:italic>
, and
<jats:italic toggle="yes">Anaerostipes</jats:italic>
) and increased levels of
<jats:italic toggle="yes">Akkermansia</jats:italic>
.
<jats:italic toggle="yes">Akkermansia</jats:italic>
abundance correlated positively with motor severity, including Hoehn–Yahr stage. Moreover,
<jats:italic toggle="yes">
nan
<jats:sub>Akk</jats:sub>
</jats:italic>
levels also correlated positively with Hoehn–Yahr stage and were significantly elevated in PD patients compared with controls. Levels in the stage 4–5 group exceeded those in the stage 1–3 group (
<jats:italic toggle="yes">P</jats:italic>
= 0.0202), indicating a stage-related increase in mucin-degrading
<jats:italic toggle="yes">
nan
<jats:sub>Akk</jats:sub>
</jats:italic>
abundance.
</jats:p>
</jats:sec>
<jats:sec>
<jats:title>Conclusions</jats:title>
<jats:p>
We have identified
<jats:italic toggle="yes">
nan
<jats:sub>Akk</jats:sub>
</jats:italic>
as a microbial gene marker associated with both the presence and severity of PD. Our qPCR-based quantification shows potential as a non-invasive biomarker for disease stratification.
</jats:p>
</jats:sec>
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