A simple alternative method for estimating hemoglobin

Edelmira Garcı́a-Nieto

doi:10.1007/s00339-025-08984-z

2025-09-29

PRE

Edelmira Garcı́a-Nieto

Libertad Juárez-Santacruz

Arely Anaya‐Hernández

J. L. Jiménez-Pérez

Ángel Netzahual-Lopantzi *

DOI：10.1007/s00339-025-08984-z

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摘要

En 中文

在本工作中，验证了一种通过红细胞上清液的热扩散率值来估算血红蛋白的替代方法。测试样本的热值被研究并与已知的血红蛋白浓度相关联。体外研究表明，红细胞与不同浓度的纳米颗粒孵育后获得血溶物；在此情况下，SiO2-Au被用作模型。血溶物中的热扩散率值范围为10.20 +/- 0.28 x 10(-4) cm(2)/s至11.98 +/- 0.14 x 10(-4) cm(2)/s。这些值结合校准曲线，允许在低浓度下（最高0.050和0.090 g/dl）定量血红蛋白。根据UV-vis和热扩散率结果，血红蛋白释放表现出线性行为。本研究表明，血溶物的热扩散率与血红蛋白浓度密切相关，使热透镜光谱技术成为细胞毒性测定未来应用的理想技术。

Keyword:

Erythrocytes

Hemolysate

SiO2-Au nanoparticles

Thermal diffusivity

Photothermal technique

期刊

Applied Physics A-Materials Science and Processing

IF:

2.8

论文数:

249

被引数:

2.8W

机构

instituto politecnico nacional - mexico

学者数:

1.6W

论文数: 1.0W

被引数: 3

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