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A Simple; Robust Method for Cellular Electrical Interfacing Using Molecular Patterning
DOI:10.1021/acsami.5c25911.png)
摘要
En 中文
通过电极周围介电表面的工程化改造,提出了一种简单且稳健的策略,用于提高电兴奋细胞培养物微电极阵列(MEA)记录的信号水平。在介电表面上引入图案化的双组分自组装单分子层(SAM)–细胞膜界面,可改善细胞与MEA之间的封接阻抗,使记录信号的幅度更接近于细胞内电位。将该技术应用于源自人诱导多能干细胞(iPSC)的心肌细胞,与传统Matrigel涂层相比,实现了近3倍的类细胞内记录产率提升,与单组分SAM相比,信号幅度提高了3倍。该技术易于应用于大多数商业和定制MEA,包括高密度互补金属氧化物半导体(CMOS)MEA,并能轻松与纳米线、微蘑菇结构以及优化的电穿孔序列相结合,以进一步增强信号水平。
Keyword:
Coating materials
Electrochemical cells
Electrodes
Interfaces
Silica
self-assembled monolayer
microelectrode array
electrophysiology
intracellular recordings
cardiomyocytes
human induced pluripotent stem cells
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