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A STED microscope aligned by design
DOI:10.1364/OE.17.016100.png)
摘要
En 中文
STED microscopes are commonly built using separate optical paths for the excitation and the STED beam. As a result, the beams must be co-aligned and can be subject to mechanical drift. Here, we present a single-path STED microscope whose beams are aligned by design and hence is insensitive to mechanical drift. The design of a phase plate is described which selectively modulates the STED beam but leaves the excitation beam unaffected. The performance of the single-beam setup is on par with previous dual-beam designs. (C) 2009 Optical Society of America
Keyword:
FLUORESCENCE MICROSCOPY
STIMULATED-EMISSION
OPTICAL VORTICES
PHASE PLATE
RESOLUTION
DIFFRACTION
GENERATION
WEDGE
BEAM
期刊
IF:
3.3
论文数:
6.1W
被引数:
14.3W
机构
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