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A sustainable strategy for biosynthesis of Rebaudioside M using bifunctional enzyme
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Baoqi WangY
Yuanyuan Ma*H
Hao Song DOI：10.1016/j.bej.2026.110233.png)
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Abstract
En 中文
• Linker composition and fusion order critically modulate hybrid enzyme activity. • UL4E hybrid enzyme exhibits superior Reb A-to-Reb M conversion efficiency. • UL4E whole-Cell Reb M production enables cost-effective synthesis. • The optimized UL4E cell system achieves 109.4% Reb M mass yield. • A sustainable strategy for production of sweetener Reb M developed.
Keywords:
bifunctional enzyme
Rebaudioside M
biosynthesis
whole-cell system
sustainable strategy
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