.png){kind=icon}
返回
A Transient Decrease in Spleen Size Following Stroke Corresponds to Splenocyte Release into Systemic Circulation
.png){kind=icon}
OA
AI
DOI：10.1007/s11481-012-9406-8.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
The splenic response to stroke is a proinflammatory reaction to ischemic injury resulting in expanded neurodegeneration. Splenectomy reduces neural injury in rodent models of hemorrhagic and ischemic stroke, however the exact nature of this response has yet to be fully understood. This study examines the migration of splenocytes after brain ischemia utilizing carboxyfluorescein diacetate succinimidyl ester (CFSE) to label them in vivo. The spleen was found to significantly decrease in size from 24 to 48 h following middle cerebral artery occlusion (MCAO) in rats compared to sham operated controls. By 96 h post-MCAO the spleen size returned to levels not different from sham operated rats. To track splenocyte migration following MCAO, spleens were injected with CFSE to label cells. CFSE positive cell numbers were significantly reduced in the 48 h MCAO group versus 48 h sham and CFSE labeled cells were equivalent in 96 h MCAO and sham groups. A significant increase of labeled lymphocyte, monocytes, and neutrophils was detected in the blood at 48 h post-MCAO when compared to the other groups. CFSE labeled cells migrated to the brain following MCAO but appear to remain within the vasculature. These cells were identified as natural killer cells (NK) and monocytes at 48 h and at 96 h post-MCAO NK cells, T cells and monocytes. After ischemic injury, splenocytes enter into systemic circulation and migrate to the brain exacerbating neurodegeneration.
Keyword:
Brain ischemia
Monocytes
Tcells
Natural killer cells
MCAO
期刊
IF:
3.5
论文数:
1.3K
被引数:
3.4K
机构
引用论文
分享收藏Design of 3.3 kW wireless battery charger for electric vehicle application considering bifurcation
PREAI
分享收藏分享收藏Allelic exclusion of mouse T cell receptor α chains occurs at the time of thymocyte TCR up-regulation.png)
Immunity
OAAI
ImmunityIF0
OAAI分享收藏Lateral fluid percussion injury of the brain induces CCL20 inflammatory chemokine expression in ratsOAAI
OAAI分享收藏Regulatory T cells are key cerebroprotective immunomodulators in acute experimental strokeNATURE MEDICINE
NATURE MEDICINEIF50
PREAI
分享收藏Inhibition of lymphocyte trafficking shields the brain against deleterious neuroinflammation after strokeBRAIN
BRAINIF11.7
PREAI
分享收藏
查看更多内容