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A turn-on fluorescent biosensor based on CdTe quantum dots for sensitive and selective detection of APE1 activity in cervical cancer cell lysates
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DOI：10.1039/D6AY00808A.png)
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Abstract
En 中文
Apurinic/apyrimidinic endonuclease 1 (APE1) plays a critical role in the base excision repair (BER) pathway; and its aberrant activity is closely associated with various cancers; including cervical cancer; as well as neurodegenerative diseases. Herein; we report a novel “turn-on” fluorescent biosensor for the sensitive and selective detection of APE1 activity based on CdTe quantum dots (QDs) and silver ions (Ag+). The sensing strategy relies on the inner filter effect (IFE)-induced fluorescence quenching of CdTe QDs by Ag+; followed by fluorescence recovery upon specific cleavage of an AP site-containing dsDNA probe by APE1. The released poly-C fragments competitively bind with Ag+; separating Ag+ from the surface of CdTe QDs and restoring the fluorescence. Under optimized conditions; the biosensor exhibits a linear response to APE1 activity in the range of 2–10 U mL−1 with a detection limit as low as 0.09 U mL−1. The sensor demonstrates excellent selectivity against interfering biomolecules; good stability; and reproducibility. Moreover; it was successfully applied to detect APE1 activity in HeLa cell lysates; showing satisfactory recovery rates. This work provides a green; simple; and robust platform for APE1 activity detection in complex biological matrices; with potential applications in cancer-related biomedical research.
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