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Absolute Quantification of Photoreceptor Outer Segment Proteins
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DOI：10.1021/acs.jproteome.3c00267.png)
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Abstract
En 中文
Photoreceptor cells generate neuronal signals in responseto capturinglight. This process, called phototransduction, takes place in a highlyspecialized outer segment organelle. There are significant discrepanciesin the reported amounts of many proteins supporting this process,particularly those of low abundance, which limits our understandingof their molecular organization and function. In this study, we usedquantitative mass spectrometry to simultaneously determine the abundancesof 20 key structural and functional proteins residing in mouse rodouter segments. We computed the absolute number of molecules of eachprotein residing within an individual outer segment and the molarratio among all 20 proteins. The molar ratios of proteins comprisingthree well-characterized constitutive complexes in outer segmentsdiffered from the established subunit stoichiometries of these complexesby less than 7%, highlighting the exceptional precision of our quantification.Overall, this study resolves multiple existing discrepancies regardingthe outer segment abundances of these proteins, thereby advancingour understanding of how the phototransduction pathway functions asa single, well-coordinated molecular ensemble.
Keywords:
vision
retina
photoreceptor
phototransduction
proteomics
absolute protein quantification
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