.png){kind=icon}
返回
Alternative splicing modulation by G-quadruplexes
.png){kind=icon}
OA
AI
DOI：10.1038/s41467-022-30071-7.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Alternative splicing is central to metazoan gene regulation, but the regulatory mechanisms are incompletely understood. Here, we show that G-quadruplex (G4) motifs are enriched similar to 3-fold near splice junctions. The importance of G4s in RNA is emphasised by a higher enrichment for the non-template strand. RNA-seq data from mouse and human neurons reveals an enrichment of G4s at exons that were skipped following depolarisation induced by potassium chloride. We validate the formation of stable RNA G4s for three candidate splice sites by circular dichroism spectroscopy, UV-melting and fluorescence measurements. Moreover, we find that sQTLs are enriched at G4s, and a minigene experiment provides further support for their role in promoting exon inclusion. Analysis of >1,800 high-throughput experiments reveals multiple RNA binding proteins associated with G4s. Finally, exploration of G4 motifs across eleven species shows strong enrichment at splice sites in mammals and birds, suggesting an evolutionary conserved splice regulatory mechanism.
Keyword:
NON-B DNA
RNA G-QUADRUPLEX
SECONDARY STRUCTURES
REGULATORY ELEMENTS
GENOME
DEPOLARIZATION
TRANSLATION
PROMOTER
REVEALS
CELLS
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
15.7
论文数:
9.3W
被引数:
91.2W
机构
引用论文
Conserved reiterated domains in Clostridium thermocellum endoglucanases are not essential for catalytic activity.png)
Gene
GeneIF0
PREAI
分享收藏Low-power and topology-free data transfer protocol with synchronous packet transmissions2014 Eleventh Annual IEEE International Conference on Sensing, Communication, and Networking (SECON)
2014 Eleventh Annual IEEE International Conference on Sensing, Communication, and Networking (SECON)IF0
PREAI
分享收藏Quantitative visualization of DNA G-quadruplex structures in human cells人类细胞中DNA G-四链体结构的定量可视化NATURE CHEMISTRY
OAAI
NATURE CHEMISTRYIF20.2
OAAI分享收藏G-quadruplex structures are stable and detectable in human genomic DNANATURE COMMUNICATIONSOAAI
NATURE COMMUNICATIONSIF15.7
OAAI分享收藏Integrative analysis reveals RNA G-quadruplexes in UTRs are selectively constrained and enriched for functional associationsNATURE COMMUNICATIONSOAAI
NATURE COMMUNICATIONSIF15.7
OAAI分享收藏Spectroscopic analysis reveals the effect of a single nucleotide bulge on G-quadruplex structures
PREAI
分享收藏Inhibition of translation in living eukaryotic cells by an RNA G-quadruplex motif通过RNA G-四链体基序抑制活真核细胞中的翻译RNAOAAI
RNAIF5
OAAI分享收藏
查看更多内容