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Amperometric biosensor for catechol using electrochemical template process

delete2011-03-01
delete26
PRE
AI
Y
Yongyan Tan
J
Jinqing Kan *
S
Shengqi Li
DOI:10.1016/j.snb.2010.12.021delete
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摘要

摘要

En 中文
A novel amperometric biosensor for the determination of catechol was developed accordingly to the electrochemical template procedure. The optimum fabricating conditions of the biosensor were studied. The resulting biosensor with the limit of less than 0.05 mu M can be used for detection of catechol in the linear range of 2.5-140 mu M. The maximum response current (I-max) and the Michaelis-Menten constant (k(m)') are 3.08 mu A and 77.52 mu M, respectively. The activation energy (E-a) of the polyphenol oxidase (PPO) catalytic reaction is 25.56 kJ mol(-1) in the B-R buffer. The stability of the PANI-CA biosensor fabricated with the electrochemical template process (retains 86% of the original activity after four months) is much higher than that fabricated with one-step and two-step processes (retains 75% of the original activity after four months). The effects of potential and pH on the response current of the biosensor are also described. (C) 2010 Elsevier B.V. All rights reserved.
Keyword:
Polyphenol oxidase
Polyaniline
Ionic liquid
Electrochemical template process
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期刊

Sensors and Actuators B-Chemical 封面图
Sensors and Actuators B-Chemical
IF:
7.7
论文数:
3.4W
被引数:
12.6W

机构

Y
Yangzhou University
学者数:
2.8W
论文数: 1.9W
被引数: 3.3W
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