.png){kind=icon}
返回
Amperometric phenol biosensor based on polyaniline
.png){kind=icon}
PRE
AI
DOI：10.1016/j.snb.2009.05.005.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
The catechol biosensor is constructed by cross-linking between polyphenol oxidase (PPO) and polyaniline (PANI) using glutaraldehyde as a cross-linking agent. The PANI, which is electrochemically synthesized in a solution containing ionic liquid, 1-ethyl-3-methylimidazolium ethyl sulfate, possesses good electroactivity and high conductivity above pH 6. In the presence of catechol as a substrate, the biosensor exhibits a linear range from 0.2 to 80 mu mol dm(-3). The maximum response current (I-max) and the Michaelis-Menten constant (k(m)') are 9.44 mu A and 117 mu mol dm(-3), respectively. The effects of pH and operating potential are also explored to optimize measurement conditions. The activation energy (E-a) of the PPO catalytic reaction is 30.23 kJ mol(-1) in the B-R buffer. Electrochemical impedance spectroscopy (EIS), UV-vis and SEM are used to characterize the PANI-PPO biosensor. The biosensor exhibits good long-term stability. (C) 2009 Elsevier B.V. All rights reserved.
Keyword:
Biosensor
Catechol
Polyphenol oxidase
Polyaniline
Ionic liquid
Cross-linking process
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
7.7
论文数:
3.4W
被引数:
12.6W
机构
引用论文
Amperometric tyrosinase based biosensor using an electrogenerated polythiophene film as an entrapment support.png)
TALANTA
OAAI
TALANTAIF6.1
OAAI分享收藏
查看更多内容