.png){kind=icon}
返回
An efficient transformation method for Bacillus subtilis DB104
.png){kind=icon}
PRE
AI
DOI：10.1007/s00253-012-3987-2.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Bacillus subtilis strains are used for extracellular expression of enzymes (i.e., proteases, lipases, and cellulases) which are often engineered by directed evolution for industrial applications. B. subtilis DB104 represents an attractive directed evolution host since it has a low proteolytic activity and efficient secretion. B. subtilis DB104 is hampered like many other Bacillus strains by insufficient transformation efficiencies (a parts per thousand currency sign10(3) transformants/mu g DNA). After investigating five physical and chemical transformation protocols, a novel natural competent transformation protocol was established for B. subtilis DB104 by optimizing growth conditions and histidine concentration during competence development, implementing an additional incubation step in the competence development phase and a recovery step during the transformation procedure. In addition, the influence of the amount and size of the transformed plasmid DNA on transformation efficiency was investigated. The natural competence protocol is easy in handling and allows for the first time to generate large libraries (1.5 x 10(5) transformants/mu g plasmid DNA) in B. subtilis DB104 without requiring microgram amounts of DNA.
Keyword:
B. subtilis DB104
Directed evolution
Natural competence
Transformation protocol
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
4.3
论文数:
1.6W
被引数:
5.4W
机构
引用论文
.png){kind=icon}
The Fluid Dynamical Performance of the Carpentier-Edwards PERIMOUNT Magna Ease Prosthesis卡彭蒂耶-爱德华兹 PERIMOUNT Magna Ease 人工瓣膜的流体动力学性能
OAAI
OAAI分享收藏
分享收藏Gibberellin A13 7-aldehyde: A proposed intermediate in the fungal biosynthesis of gibberellin A3
PREAI
分享收藏
查看更多内容