.png){kind=icon}
.png){kind=icon}
Return
An indole-oxadiazole-derived fluorescent probe for the detection of the human serum albumin level in thoracic surgery
.png){kind=icon}
PRE
AI
H
G
Y
L
J
Y
DOI：10.1016/j.ab.2026.116090.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
In this work, the fluorescent probe InOx-HSA was developed from an indole-oxadiazole derivative for the detection of the HSA level in thoracic surgery. The probe reported a ratio fluorescence response from 405 nm to 505 nm under the excitation at 310 nm. InOx-HSA occupied the binding site of phenylbutazone in HSA and generated the fluorescence enhancement via Fo & uml;rster resonance energy transfer/twisted intramolecular charge transfer mechanisms. The response between InOx-HSA and HSA completed within 30 min. The probe-HSA complex kept stable within the range of 7.0-9.0. The ratio response was in a dosage-dependent manner with the limit of detection as 0.0002 mg/mL. InOx-HSA exhibited high specificity and low cytotoxicity. The site-binding strategy was confirmed by the drug competition, trypsin digestion, and protein denaturation by high temperature. InOx-HSA achieved visualizing HSA in living pulmonary cells, and revealed the ventilation-related consumption of HSA during the thoracic surgery.
Keywords:
Human serum albumin
Fluorescence probe
Thoracic surgery
Intracellular imaging
Hypoxia condition
Journal
IF:
2.5
Papers:
272
Citations:
3.3W
Organization
Cited Papers