.png){kind=icon}
.png){kind=icon}
Return
An outbreak of human astrovirus type 5 in a kindergarten in Chongqing, Southwest China
.png){kind=icon}
OA
AI
L
J
S
C
C
B
Y
X
DOI：10.1186/s12879-026-14118-4.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
On April 20th, 2025, the local Center for Disease Control and Prevention (CDC) received a report regarding several kindergarteners exhibiting symptoms, including vomiting, diarrhoea and fever. Epidemiological investigations and laboratory testing were conducted to identify the causes, formulate preventive and control measures and provide guidance to identify, investigate and manage similar events. An on-site epidemiological investigation was conducted to characterise the clinical and epidemiological features of these cases. Anal swabs from patients and samples from relevant personnel and environmental sources were collected for etiological investigation. The presence of pathogens was confirmed in all anal swabs by multiplex real-time polymerase chain reaction test. Genotyping was performed by sequencing and phylogenetic analysis. Nineteen cases met the case definition, with an attack rate of 43.18%. The main clinical manifestations were fever (100%), followed by abdominal pain (68.42%), and diarrhoea (42.11%). Amongst the eight sampled kindergarteners, seven were positive for human astrovirus (HAstV). Whole-genome sequencing and bioinformatics analysis revealed that the outbreak strain belonged to genotype 5 and the strain sequences of the positive cases were highly homologous (p distance < 0.001). Phylogenetic analysis showed that this strain was closely related to the previously prevalent HAstV-5 strain isolated from Fuzhou, China in 2013 (GenBank accession no. MF684776.1). No gene recombination events were found. According to the laboratory test results, HAstV-5 was the aetiological agent of the outbreak. Further research focusing on enhanced surveillance strategies and improved diagnostic tools is required to guide the identification, investigation and management of HAstV outbreaks. Not applicable.
Keywords:
Human astrovirus
Kindergarten
Epidemiological investigation
Molecular epidemiology
Journal
IF:
3
Papers:
1.5W
Citations:
3.0W
Organization
Cited Papers