.png){kind=icon}
.png){kind=icon}
Return
Antifungal efficacy of three brown seaweed tannins on Fusarium oxysporum growth, reproduction, and cellular enzymes
.png){kind=icon}
PRE
AI
G
K
K
DOI：10.1080/15569543.2026.2633138.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Among the variety of biologically active phytocompounds, polyphenols such as tannin have been found to effectively inhibit the growth of Fusarium oxysporum. Using a range of assays, the effects of gallic and tannic acid as well as seaweed crude tannin extracts (CTE) and their column chromatographic fractions 1 and 2 were investigated.
Extraction of the tannin from seaweeds and separation using a Sephadex LH-20 column and thin-layer chromatography, then identifying the bioactive principles through GC-MS, and determination of the level of tannin using an HPLC C18 column can be accomplished. The antifungal activity was determined using diffusion bioassay, minimum inhibitory concentration (MIC), and conidial population count of F. oxysporum. The mechanism of action of the tannins was recorded by measuring the levels of F. oxysporum mycelial cellulase, amylase, protease, lipase, and xylanase.
Tannic acid has greater effects than gallic acid. Turbinaria ornata CTE significantly reduced the growth of F. oxysporum. Stoechospermum marginatum tannin fraction 1 showed the highest level of antifungal activity. For crude and fractions, the MIC values are 0.6 and 0.12%, respectively. F. oxysporum growth curve lacks a stationary phase. In comparison with brown algal crude tannins, the development of F. oxysporum conidia was dramatically inhibited by gallic acid and tannic acids. When compared to other tannin fractions that were assessed, conidial production was significantly reduced by Sargassum wightii tannin F2. The results demonstrated a dose-dependent reduction of enzyme activities of F. oxysporum by seaweeds CTE. At higher concentrations, T. ornata significantly reduced amylase and pectinase activities, whereas S. wightii reduced protease, cellulase, and xylanase activities, and S. marginatum reduced lipase activity.
To reduce cotton wilt in the field, use column chromatographic fractions of CTE from S. wightii, S. marginatum, and T. ornata in the near future.
Keywords:
Seaweeds
tannin
toxicity
Fusarium oxysporum
zone of inhibition
minimum inhibitory concentration
Journal
IF:
2.4
Papers:
837
Citations:
1.8K
Organization
Cited Papers