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Ascorbic acid biosensing methods: A review
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DOI：10.1016/j.procbio.2022.03.028.png)
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Ascorbic acid has a significant regulatory effect on neurotransmitters and enzymes in the central nervous system. In apparently healthy people, the reference range for ascorbic acid is 0.6-2 mg/dL. The variable concentration of ascorbic acid within biology fluids was found in clinical investigations to be a metric for assessing the exact amount of oxidative stress in the body's metabolism. The rise in oxidative stress is well-defined in connection with diabetes, cancer, and liver disease. There are various methods for detecting ascorbic acid which are timeconsuming, necessitate sample pre-treatment and require high-cost instrumental set-up. These disadvantages were overcome by biosensing methods, as these are precise, rapid, simple, economical, and extremely sensitive. The classification, operational principles, merits and demerits of ascorbate biosensors, based on transducers, is described in this review article. Biosensors for ascorbic acid performed optimally in the pH range of 1-7.4, at room temperature, and in the ascorbic acid concentration range of 0.005-18,000 mu M, with a minimum detection limit of 0.008 mu M, at a working potential of 0.05-3.8 V. Ascorbic acid levels in blood, serum, and urine were monitored using these biosensors. Ascorbate biosensors' economic advancement and commercialisation are suggested.
Keyword:
Ascorbic acid
Vitamin C
Ascorbic acid biosensors
Nanomaterials
Biological fluids
Oxidative stress
期刊
IF:
4
论文数:
8.1K
被引数:
2.0W
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