.png){kind=icon}
返回
Auto-Align - Multi-Modality Fluorescence Microscopy Image Co-registration
.png){kind=icon}
OA
AI
DOI：10.1111/j.1600-0854.2011.01309.x.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Multi-modality microscopes incorporate multiple microscopy techniques into one module, imaging through a common objective lens. Simultaneous or consecutive image acquisition of a single specimen, using multiple techniques, increases the amount of measurable information available. In order to benefit from each modality, it is necessary to accurately co-register data sets. Intrinsic differences in the image formation process employed by each modality result in images which possess different characteristics. In addition, as a result of using different measurement devices, images often differ in size and can suffer relative geometrical deformations including rotation, scale and translation, making registration a complex problem. Current methods generally rely on manual input and are therefore subject to human error. Here, we present an automated image registration tool for fluorescence microscopy. We show that it successfully registers images obtained via total internal reflection fluorescence (TIRF), or epi-fluorescence, and confocal microscopy. Furthermore, we provide several other applications including channel merging following image acquisition through an emission beam splitter, and lateral stage drift correction. We also discuss areas of membrane trafficking which could benefit from application of Auto-Align. Auto-Align is an essential item in the advanced microscopist's toolbox which can create a synergy of single or multi-modality image data.
Keyword:
fluorescence microscopy
image analysis
image registration
stage drift
time lapse
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
2.5
论文数:
2.4K
被引数:
5.8K
机构
引用论文
分享收藏分享收藏Response of Bean and Tobacco to Ozone: Effect of Light Intensity, Temperature and Relative Humidity
PREAI
分享收藏分享收藏A technical review on gas diffusion, mechanism and medium of PEM fuel cellPEM燃料电池气体扩散、机理及介质技术综述.png)
Ionics
IonicsIF0
PREAI
分享收藏
查看更多内容