Automated Regularized Deconvolution for Eliminating Extra-Column Effects in Fast High-Efficiency Separations

Troy T. Handlovic

doi:10.1021/acs.analchem.3c01279

2023-07-10

PRE

Daniel W. Armstrong *

DOI：10.1021/acs.analchem.3c01279

原文链接

原文求助

摘要

En 中文

With the introduction of ultrahighefficiency columnsand fastseparations, the need to eliminate peak deformation contributed bythe instrument must be effectively solved. Herein, we develop a robustframework to automate deconvolution and minimize its artifacts, suchas negative dips, wild noise oscillations, and ringing, by combiningregularized deconvolution and Perona-Malik (PM) anisotropicdiffusion methods. A asymmetric generalized normal (AGN) functionis proposed to model the instrumental response for the first time.With no-column data at various flow rates, the interior point optimizationalgorithm extracts the parameters describing instrumental distortion.The column-only chromatogram was reconstructed using the Tikhonovregularization technique with minimal instrumental distortion. Forillustration, four different chromatography systems are used in fastchiral and achiral separations with 2.1 and 4.6 mm i.d. columns. OrdinaryHPLC data can approach highly optimized UHPLC data. Similarly, infast HPLC-circular dichroism (CD) detection, 8000 plates were gainedfor a fast chiral separation. Moment analysis of deconvolved peaksconfirms correction of the center of mass, variance, skew, and kurtosis.This approach can be easily integrated and used with virtually anyseparation and detection system to provide enhanced analytical data.

Keyword:

CHROMATOGRAPHY

期刊

Analytical Chemistry

IF:

6.7

论文数:

4.7W

被引数:

15.9W

机构

university of texas system

学者数:

18.5W

论文数: 15.6W

被引数: 210

引用论文

Low-intensity exercise training decreases cardiac output and hypertension in spontaneously hypertensive rats

American Journal of Physiology-Heart and Circulatory Physiology

IF0

1997-12-01

PREAI

Acácio Salvador Véras-Silva; Katt Coelho Mattos; Nilo Sérgio Gava; Patricia Chakur Brum; Carlos Eduardo Negrão; Eduardo Moacyr Krieger

Role of body mass index on total knee replacement rehabilitation and outcome

National Journal of Clinical Orthopaedics

IF0

2020-10-01

OAAI

Dr. Seelam Narasimha Reddy; Dr. Buggaveeti Goutham; Dr. Yadlapalli Venkatesh

Extraction of intrinsic column peak profiles of narrow-bore and microbore columns by peak deconvolution methods

ANALYTICA CHIMICA ACTA

IF6

2021-10-01

PREAI

Gritti, Fabrice; Wahab, Farooq

Extra-column band broadening effects in contemporary liquid chromatography: Causes and solutions

TRAC-TRENDS IN ANALYTICAL CHEMISTRY

IF12

2019-10-01

OAAI

Desmet, Gert; Broeckhoven, Ken

Current challenges and future prospects in chromatographic method development for pharmaceutical research

TRAC-TRENDS IN ANALYTICAL CHEMISTRY

IF12

2017-10-01

104

PREAI

Mattrey, Frederick T.; Makarov, Alexey A.; Regalado, Erik L.; Bernardoni, Frank; Figus, Margaret; Hicks, Michael B.; Zheng, Jinjian; Wang, Lin; Schafer, Wes; Antonucci, Vincent; Hamilton, Simon E.; Zawatzky, Kerstin; Welch, Christopher J.

Eye Movements during Reading

Optometry and Vision Science

IF0

1976-08-01

PREAI

Kenneth J. Ciuffreda; A. Terry Bahill; Robert V. Kenyon; Lawrence Stark

The clinical outcomes of cementless unicompartmental knee replacement in patients with reduced bone mineral density

Journal of Orthopaedic Surgery and Research

IF0

2020-01-31

OAAI

Hasan R. Mohammad; James A. Kennedy; Stephen J. Mellon; Andrew Judge; Christopher A. Dodd; David W. Murray