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Aza-Paternò–Büchi derivatization enables annotation of triacylglycerols by negative-ion-mode mass spectrometry at the double bond level
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DOI：10.1016/j.talanta.2026.130396.png)
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Abstract
En 中文
• First negative-ion-mode workflow for TG double-bond annotation by LC-HRMS. • Aza-Paternò–Büchi derivatization enables TG CC isomer resolution. • Solvent composition and reaction kinetics were systematically optimized. • Charge localization governs diagnostic fragmentation of derivatized TGs. • TG regioisomers were profiled in plasma and vegetable seed oils.
Keywords:
Lipidomics
High-resolution mass spectrometry
Regioisomer
Carbon-carbon double bonds
Seed oil
Plasma
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