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摘要
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Biosynthetic pathways of brassinolide from campesterol was demonstrated by studies using cultured Catharanthus roseus cells. Brassinolide is biosynthesized through two pathways, early C6-oxidation pathway and late C6-oxidation pathway, branching off at the conversion of campestanol. Recent characterization of brassinosteroid-deficient mutants of Arabidopsis, pea and tomato confirmed that the pathways operate in wide variety of plant species. Biochemical and molecular genetic studies of the mutants are providing important knowledge on genes and enzymes involved in brassinosteroid biosynthesis. The established biosynthetic pathways of brassinosteroids and the regulation of biosynthesis including up-to-date findings are introduced in this review. (C) Elsevier, Paris.
Keyword:
brassinosteroids
biosynthesis
brassinosteroid-deficient mutants
Catharanthus roseus
cell culture system
early C6-oxidation pathway
late C6-oxidation pathway
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3.4W
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