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Calcium as a molecular switch: Gentle antibody-purification through directed evolution of Protein G
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DOI：10.1016/j.seppur.2026.138115.png)
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Abstract
En 中文
• Engineering of Protein G for affinity capture of antibodies via interaction with the Fab-fragment. • Affinity purification of full-length monoclonal mouse antibodies is presented. • Mild elution from the resin at neutral pH (7.0) is possible. • The structure-function relationship of the calcium-dependent elution mechanism is described. • A valuable tool for purification of acid-sensitive antibodies and Fab-fragments is developed.
Keywords:
Mild affinity purification
Protein engineering
Protein G
Switch
NMR
Calcium-dependency
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