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Calcium Uncaging with Visible Light
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DOI：10.1021/jacs.5b11606.png)
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摘要
En 中文
We have designed a nitroaromatic photochemical protecting group that absorbs visible light in the violet-blue range. The chromophore is a dinitro derivative of bisstyrylthiophene (or BIST) that absorbs light very effectively (epsilon(440) = 66,000 M-1 cm(-1) and two-photon cross section of 350 GM at 775 nm). We developed a caged calcium molecule by conjugation of BIST to a Ca2+ chelator that upon laser flash photolysis rapidly releases Ca2+ in <0.2 ms. Using the patch clamp method the optical probe, loaded with Ca2+ was delivered into acutely isolated mouse cardiac myocytes, where either one- and two-photon uncaging of Ca2+ induced highly local or cell-wide physiological Ca2+ signaling events.
Keyword:
PHOTOREMOVABLE PROTECTING GROUPS
BETA-ADRENERGIC STIMULATION
LASER FLASH-PHOTOLYSIS
PIG CARDIAC MYOCYTES
CAGED CALCIUM
2-PHOTON ABSORPTION
CAGING GROUP
NITROPHENYL-EGTA
CROSS-SECTIONS
IN-VIVO
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期刊
IF:
15.6
论文数:
20.0W
被引数:
60.2W
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引用论文
Laser photolysis of caged calcium: Rates of calcium release by nitrophenyl-EGTA and DM-nitrophen笼中钙的激光光解: 硝基苯基-EGTA和DM-硝基酚释放钙的速率.png)
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BIOPHYSICAL JOURNALIF3.1
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