Capacious and programmable multi-liposomal carriers

doi:10.1039/c4nr06037g

Capacious and programmable multi-liposomal carriers

2015-01-01

31

PRE

AI

A

Alexander A. Yaroslavov *

Vyacheslav V. Samoshin

DOI：10.1039/c4nr06037g

Original

Original request for help

Save

Abstract

En 中文

Spherical polycationic brushes (SPBs) were synthesized by grafting polycationic chains onto 100 nm polystyrene particles. These particles were exposed to unilamellar egg-lecithin (EL) liposomes with a mean diameter of 40 nm that had been rendered anionic via the presence of 10 molar% of phosphatidylserine (PS1-). The liposomes also contained 30 mole% of a morpholinocyclohexanol-based lipid (MOCH) that undergoes a conformational flip when the pH is decreased from 7.0 to 5.0. Mixtures of SPBs and liposomes at pH 7 gave an electrostatically-driven complex possessing, on average, about 40 liposomes for each SPB particle. It was found that the bound liposomes rapidly release much of their contents when the pH is reduced from 7.0 to 5.0 owing mostly to a MOCH conformational change that creates defects in the bilayer membrane. The drop in pH does not, however, induce a separation of the liposomes from the SPBs. Around 50-60% of the liposome contents escape before, it is reasoned, lateral and transmembrane motion of the membrane components heals the defects and prevents further release. Remarkably, the liposomes complexed with SPB release their cargo much faster than the identical but non-complexed liposomes.

Keywords:

IN-VITRO

DRUG-DELIVERY

GENE DELIVERY

PH

POLYELECTROLYTE

RELEASE

POLYMER

MICROENVIRONMENT

STRATEGIES

Capacious and programmable multi-liposomal carriers

Abstract

AI Summary

Journal

Organization