Cell growth on 3D microstructured surfaces

W. W. R. Araújo

doi:10.1016/j.msec.2016.03.026

2016-06-01

W. W. R. Araújo *

F. S. Teixeira

Glenda Nicioli da Silva

Daisy Maria Fávero Salvadori

M. C. Salvadori

I.G. Brown

DOI：10.1016/j.msec.2016.03.026

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摘要

En 中文

Chinese Hamster Ovary (CHO) cell cultures were grown on surfaces lithographed with periodic 3D hexagonal microcavity array morphology. The range of microcavity size (inscribed circle diameter) was from 12 mu m to 560 mu m. CHO cells were grown also on flat surfaces. The characterization was performed with respect to cell growth density (number of nuclei per unit area) by fluorescence optical microscopy and evaluated by correlation function analysis. We found that optimum microcavity radius was 80 mu m, concerning to the maximum cell growth density, being even greater than the growth density on a flat (unstructured) substrate of the same material. This finding can be important for optimization of biotechnological processes and devices. (C) 2016 Published by Elsevier B.V.

Keyword:

Biomaterials

Surface patterning

Cell aggregation

Optical microscopy

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Materials Science and Engineering C-Materials for Biological Applications

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8.1

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6.0K

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Universidade Estadual Paulista

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universidade de sao paulo

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