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Combination of different preservation techniques as low-cost strategies inhibiting sugar degradation in liquid feedstock used for bioethanol fermentation
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PRE
AI
J
J
J
J
DOI：10.1016/j.biombioe.2022.106655.png)
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Abstract
En 中文
Just like for the food supply chain, sugar-based feedstock used in the bioethanol industry might be affected by biological contamination, resulting in important economic losses. Therefore, it is necessary to develop costeffective storage techniques capable of preserving feedstock over several months. In this study, three different techniques (ozonation, acidification, and addition of a corn oil layer) were investigated individually as well as combined for the long-term storage of sugars dedicated to fermentation. After 300 days of storage, up to 100% of the initial fermentable sugars were preserved with acidification (pH 2.0), independently or in conjunction with other conservation techniques. However, in terms of fermentation potential, the groups treated with acid had a lower ethanol yield than the groups maintained at pH 6.0. When the solution was treated with ozone combined with acidification, it yielded 55.71% of bioethanol efficiency, whereas when treated only with ozonation at pH 6.0, fermentation reached a 67.20% yield. Ozonation was found to be efficient to preserve fermentable sugars, either with the use of an oil layer (80% conservation) or at lower pH (100% ethanol efficiency). Finally, the combination of ozonation and an oil layer presented the highest total ethanol production, reaching 101g L-1 after 96h of incubation using Saccharomyces cerevisiae. Thus, the combined use of ozonation and an oil layer has the potential to be used in the bioethanol sector as a system to preserve liquid feedstock without the use of evaporation to increase sugar concentration.
Keywords:
Sugar beet molasses
Fermentation
Bioethanol
Ozone
Oil layer