COVID-19: detection methods in post-mortem samples

Ilaria Tedesco

doi:10.32074/1591-951X-933

2023-10-01

Ilaria Tedesco

Federica Zito Marino

Andrea Ronchi

Amaro Nunes Duarte Neto

Marisa Dolhnikoff

Maurizio Municinò

Carlo Pietro Campobasso

Giuseppe Pannone

Renato Franco *

DOI：10.32074/1591-951X-933

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摘要

En 中文

COVID-19 identification is routinely performed on fresh samples, such as nasopharyngeal and oropharyngeal swabs, even if, the detection of the virus in formalin-fixed paraffinembedded (FFPE) autopsy tissues could help to underlie mechanisms of the pathogenesis that are not well understood. The gold standard for COVID-19 detection in FFPE samples remains the qRT-PCR as in swab samples, contextually other methods have been developed, including immunohistochemistry (IHC), and in situ hybridization (ISH). In this manuscript, we summarize the main data regarding the methods of COVID-19 detection in pulmonary and extra-pulmonary post-mortem samples, and especially the sensitivity and specificity of these assays will be discussed.

Keyword:

COVID-19

immunohistochemistry

in situ hybridization

autopsy samples

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Pathologica

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University of Foggia

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universidade de sao paulo

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universita della campania vanvitelli

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