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CRISPR-Cas12a based aptasensor for sensitive and selective ATP detection
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DOI：10.1016/j.snb.2020.128164.png)
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摘要
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CRISPR-Cas based detection platform opens up a new avenue for biosensing owing to its programmability, sequence specificity and high base resolution upon nucleic acid targets. In current study, we utilised the target DNA-induced non-specific single-stranded DNA cutting activity (trans-cleavage) of CRISPR-Cas12a to fabricate an aptamer mediated fluorescent biosensor for sensitive and selective detection of adenosine triphosphate (ATP). In this sensor, we designed an ATP-binding aptamer as the target ssDNA for Cas12a, thereby in the presence of ATP, the ATP-binding aptamer would be occupied by ATP, and less target ssDNA can be processed by Cas12a, which gave rise to an ATP concentration dependent change in fluorescence resulted from trans-cleavage of doubly labeled ssDNA reporter. In this way, we successfully translated ATP signals to nucleic acid signals that can be amplified by Cas12a process. The dynamic detection range was from 1 mu M to 200 mu M and the limit of detection was 400 nM. The entire sample-to-answer time for this biosensor was around 40 min. Overall, this novel biosensor balanced sensitivity, specificity, detection time and ease of use. Our proposed biosensor provided a principle-of-proof for detecting small molecule by using CRISPR-Cas12a system, which would unlock its potential and further its futuristic applications in the field of diagnostics.
Keyword:
CRISPR-Cas12a
Trans-cleavage
Small molecule detection
ATP
Aptamer
Fluorescent biosensor
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7.7
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3.4W
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12.6W
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