.png){kind=icon}
返回
Dermal cells distribution on laser-structured ormosils
.png){kind=icon}
OA
AI
DOI：10.1002/term.507.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Several dermal substitutes for skin grafting are now commercially available, although their performance still needs improvement. Most artificial dermises have a lower take rate than autologous grafts and require more time for sufficient vascular ingrowth to overlay the skin graft. Herein we characterize new two-dimensional scaffolds for tissue-engineering applications, which were fabricated by two-photon polymerization (2PP) of ormosils hybrid materials. For the 2PP experiments, a Ti:sapphire laser was used to induce the photopolymerization. In this study we showed that the polymeric structures with controlled architectures produced via 2PP could be used as scaffolds for the in vitro culture and proliferation of human dermal fibroblasts. Fluorescence microscopy revealed that the fibroblasts' orientation was guided by the scaffold geometry, consisting of ormosils lines or grids. This 'dermal equivalent' was investigated for its ability to accommodate epidermal cells. To evaluate this interaction, two experimental approaches were hence used: (a) fibroblastmelanocyte co-cultures; and (b) fibroblastkeratinocyte organotypic cultures. During their growth on ormosil scaffolds, productive interaction of fibroblasts with both epidermal cell types was found. Moreover, this pseudo-dermis was shown to support the growth of keratinocytes for up to 8days after their seeding. Copyright (c) 2011 John Wiley & Sons, Ltd.
Keyword:
dermal grafts
ormosils scaffolds
two-photon polymerization
fibroblast
melanocyte
keratinocyte co-cultures
tissue engineering
biocompatible
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
2.6
论文数:
2.3K
被引数:
5.5K
机构
引用论文
Fabrication and test of sol-gel based planar oxygen optodes for use in aquatic sediments.png)
MARINE CHEMISTRY
MARINE CHEMISTRYIF2.5
PREAI
分享收藏The use of split-thickness dermal grafts to resurface full thickness skin defects使用分厚真皮移植物来修复全厚度皮肤缺损BURNS
BURNSIF2.9
PREAI
分享收藏
查看更多内容