.png){kind=icon}
返回
Developing a biomimetic tooth bud model
.png){kind=icon}
OA
AI
DOI：10.1002/term.2246.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
A long-term goal is to bioengineer, fully functional, living teeth for regenerative medicine and dentistry applications. Biologically based replacement teeth would avoid insufficiencies of the currently used dental implants. Using natural tooth development as a guide, a model was fabricated using post-natal porcine dental epithelial (pDE), porcine dental mesenchymal (pDM) progenitor cells, and human umbilical vein endothelial cells (HUVEC) encapsulated within gelatin methacrylate (GelMA) hydrogels. Previous publications have shown that post-natal DE and DM cells seeded onto synthetic scaffolds exhibited mineralized tooth crowns composed of dentin and enamel. However, these tooth structures were small and formed within the pores of the scaffolds. The present study shows that dental cell-encapsulated GelMA constructs can support mineralized dental tissue formation of predictable size and shape. Individually encapsulated pDE or pDM cell GelMA constructs were analysed to identify formulas that supported pDE and pDM cell attachment, spreading, metabolic activity, and neo-vasculature formation with co-seeded endothelial cells (HUVECs). GelMa constructs consisting of pDE-HUVECS in 3% GelMA and pDM-HUVECs within 5% GelMA supported dental cell differentiation and vascular mineralized dental tissue formation in vivo. These studies are the first to demonstrate the use of GelMA hydrogels to support the formation of post-natal dental progenitor cell-derived mineralized and functionally vascularized tissues of specified size and shape. These results introduce a novel three-dimensional biomimetic tooth bud model for eventual bioengineered tooth replacement teeth in humans. Copyright (c) 2017 John Wiley & Sons, Ltd.
Keyword:
biodegradable hydrogel
cell encapsulation
cell differentiation
biomineralization
progenitor cell
vascularization
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
2.6
论文数:
2.3K
被引数:
5.5K
机构
引用论文
Rational design of hydrogels for tissue engineering: Impact of physical factors on cell behavior.png)
BIOMATERIALS
BIOMATERIALSIF12.9
PREAI
分享收藏Does digitalisation policy really help with corporate CE Evidence from Chinese manufacturing companies数字化政策是否真的有助于中国制造公司的企业CE证据
PREAI
分享收藏Engineering robust and functional vascular networks in vivo with human adult and cord blood-derived progenitor cells用成人和脐带血来源的祖细胞在体内构建强大而功能的血管网络CIRCULATION RESEARCHOAAI
CIRCULATION RESEARCHIF16.2
OAAI分享收藏Plaque Progression Differences Between Apixaban and Rivaroxaban in Patients With Atrial Fibrillation Measured With Cardiac Computed Tomography and Plaque Quantification心房颤动患者中阿哌沙班与利伐沙班在斑块进展方面的差异（通过心脏计算机断层扫描和斑块量化测量）
PREAI
分享收藏分享收藏
查看更多内容