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Dielectrophoretic microbead sorting using modular electrode design and capillary-driven microfluidics
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DOI：10.1007/s10544-017-0238-4.png)
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Multiplexing assays using microbeads in microfluidics offers high flexibility and throughput, but requires the ability to sort particles based on their physical properties. In this paper, we present a continuous method for separating microbeads that is compact, modular and adaptive, employing an optimized electrode layout that alternates sorting and concentration of microbeads using dielectrophoresis and a nested design. By simulating the combined effects of the hydrodynamic drag and dielectrophoresis forces on polystyrene beads, the parameters of the electrode layout and voltage configuration are optimized for maximum separation based on particle size with a small number of slanted planar electrodes. Experimental verification confirms the efficient separation of 10 mu m and 5 mu m beads, with similar to 98% of all concentrated beads sorted in two separate streams and only similar to 2% of 5 mu m beads leaking into the 10 mu m bead stream. In addition, this method is implemented on capillary-driven microfluidic chips for maximum portability and ease of use.
Keyword:
Microfluidic
Microbeads
Dielectrophoresis
Particle sorting
Lab on a chip
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