Differential Phase Contrast Imaging to Predict MSC Immune Function

Kejie Rui

doi:10.1002/adhm.202501553

2025-07-02

Kejie Rui

Priyanka Priyadarshani

Carlos Muñoz

Steven L. Stice

Luke J. Mortensen *

DOI：10.1002/adhm.202501553

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摘要

En 中文

间充质基质细胞（MSCs）表现出显著的免疫调节潜力，使其成为自身免疫和炎症性疾病细胞治疗的有希望的候选者。然而，MSC培养物的异质性和缺乏稳健的、可预测的效力测定方法阻碍了其临床转化。本研究探讨了在MSC扩增过程中单细胞形态学成像的潜力，作为一种估算色氨酸-2,3-双加氧酶（IDO）蛋白和酶活性的方法，这是一种常见的免疫抑制能力指标。通过荧光和无标记定量差分相位对比（qDPC）成像，在生物制造过程中非侵入性地提取活体MSC的形态学特征，并利用机器学习（ML）回归模型预测单个细胞的IDO活性。qDPC成像表征被扩展以估算基于其IDO活性和对T细胞的免疫抑制作用的先前建立的MSC效力共识模型。这些发现为可扩展、非破坏性的MSC免疫调节能力监测奠定了基础，促进了MSC制造和质量控制的未来发展战略以及临床应用的开发。

Keyword:

cell morphology

immunosuppressive capacity

Mesenchymal stromal cells

quantitative differentiation phase contrast imaging

期刊

Advanced Healthcare Materials

IF:

9.6

论文数:

7.7K

被引数:

4.4W

机构

University of Georgia

学者数:

1.5W

论文数: 1.2W

被引数: 2.9W

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