.png){kind=icon}
返回
Diffusion Mapping with Diffractive Optical Elements for Periodically Patterned Photobleaching
.png){kind=icon}
PRE
AI
DOI：10.1021/acs.analchem.3c05728.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Fourier transform-fluorescence recovery after photobleaching (FT-FRAP) using a diffractive optical element (DOE) is shown to support distance-dependent diffusion analysis in biologically relevant media. Integration of DOEs enables patterning of a dot array for parallel acquisition of point-bleach FRAP measurements at multiple locations across the field of view. In homogeneous media, the spatial harmonics of the dot array analyzed in the spatial Fourier transform domain yield diffusion recovery curves evaluated over specific well-defined distances. Relative distances for diffusive recovery in the spatial Fourier transform domain are directly connected to the 2D (h,k) Miller indices of the corresponding lattice lines. The distribution of the photobleach power across the entire field of view using a multidot array pattern greatly increases the overall signal power in the spatial FT-domain for signal-to-noise improvements. Derivations are presented for the mathematical underpinnings of FT-FRAP performed with 2D periodicity in the photobleach patterns. Retrofitting of FT-FRAP into instrumentation for high-throughput FRAP analysis (Formulatrix) supports automated analysis of robotically prepared 96-well plates for precise quantification of molecular mobility. Figures of merit are evaluated for FT-FRAP in analysis for both slow diffusion of fluorescent dyes in glassy polymer matrices spanning several days and model proteins and monoclonal antibodies within aqueous solutions recovering in matters of seconds.
Keyword:
FLUORESCENCE RECOVERY
ANOMALOUS DIFFUSION
LCP-FRAP
MACROMOLECULES
SCIENCES
RELEASE
PLANE
ASSAY
期刊
IF:
6.7
论文数:
4.7W
被引数:
15.9W
机构
引用论文
Periodic Photobleaching with Structured Illumination for Diffusion Imaging用于扩散成像的结构化照明的周期性光漂白.png)
ANALYTICAL CHEMISTRY
ANALYTICAL CHEMISTRYIF6.7
PREAI
分享收藏Anomalous Diffusion Characterization by Fourier Transform-FRAP with Patterned IlluminationBIOPHYSICAL JOURNAL
OAAI
BIOPHYSICAL JOURNALIF3.1
OAAI分享收藏
查看更多内容