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Digital staining in optical microscopy using deep learning - a review
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DOI：10.1186/s43074-023-00113-4.png)
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摘要
En 中文
Until recently, conventional biochemical staining had the undisputed status as well-established benchmark for most biomedical problems related to clinical diagnostics, fundamental research and biotechnology. Despite this role as gold-standard, staining protocols face several challenges, such as a need for extensive, manual processing of samples, substantial time delays, altered tissue homeostasis, limited choice of contrast agents, 2D imaging instead of 3D tomography and many more. Label-free optical technologies, on the other hand, do not rely on exogenous and artificial markers, by exploiting intrinsic optical contrast mechanisms, where the specificity is typically less obvious to the human observer. Over the past few years, digital staining has emerged as a promising concept to use modern deep learning for the translation from optical contrast to established biochemical contrast of actual stainings. In this review article, we provide an in-depth analysis of the current state-of-the-art in this field, suggest methods of good practice, identify pitfalls and challenges and postulate promising advances towards potential future implementations and applications.
Keyword:
Deep learning
Digital staining
Optical microscopy
Virtual staining
In-silica
Pseudo-H& E
Virtual fluorescence
Generative models
Image translation
期刊
IF:
19.1
论文数:
286
被引数:
2.3K
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