.png){kind=icon}
返回
Digitally programmable microfluidic automaton for multiscale combinatorial mixing and sample processing
.png){kind=icon}
OA
AI
DOI：10.1039/c2lc40861a.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
A digitally programmable microfluidic Automaton consisting of a 2-dimensional array of pneumatically actuated microvalves is programmed to perform new multiscale mixing and sample processing operations. Large (mu L-scale) volume processing operations are enabled by precise metering of multiple reagents within individual nL-scale valves followed by serial repetitive transfer to programmed locations in the array. A novel process exploiting new combining valve concepts is developed for continuous rapid and complete mixing of reagents in less than 800 ms. Mixing, transfer, storage, and rinsing operations are implemented combinatorially to achieve complex assay automation protocols. The practical utility of this technology is demonstrated by performing automated serial dilution for quantitative analysis as well as the first demonstration of on-chip fluorescent derivatization of biomarker targets (carboxylic acids) for microchip capillary electrophoresis on the Mars Organic Analyzer. A language is developed to describe how unit operations are combined to form a microfluidic program. Finally, this technology is used to develop a novel microfluidic 6-sample processor for combinatorial mixing of large sets (>2(6) unique combinations) of reagents. The digitally programmable microfluidic Automaton is a versatile programmable sample processor for a wide range of process volumes, for multiple samples, and for different types of analyses.
Keyword:
CAPILLARY-ELECTROPHORESIS MICRODEVICE
CARBONACEOUS BIOMARKERS
CHIP
CAPTURE
DILUTE
VALVES
期刊
L
IF:
5.4
论文数:
9.0K
被引数:
3.3W
机构
引用论文
Analysis of carbonaceous biomarkers with the Mars Organic Analyzer microchip capillary electrophoresis system: Aldehydes and ketones.png)
ELECTROPHORESIS
ELECTROPHORESISIF2.5
PREAI
分享收藏Toward Total Automation of Microfluidics for Extraterrestial In Situ AnalysisANALYTICAL CHEMISTRY
ANALYTICAL CHEMISTRYIF6.7
PREAI
分享收藏
Integrated affinity capture, purification, and capillary electrophoresis microdevice for quantitative double-stranded DNA analysisANALYTICAL CHEMISTRY
ANALYTICAL CHEMISTRYIF6.7
PREAI
分享收藏Enhanced Amine and Amino Acid Analysis Using Pacific Blue and the Mars Organic Analyzer Microchip Capillary Electrophoresis SystemANALYTICAL CHEMISTRY
ANALYTICAL CHEMISTRYIF6.7
PREAI
分享收藏
查看更多内容