.png){kind=icon}
返回
Direct protein microarray fabrication using a hydrogel stamper
.png){kind=icon}
PRE
AI
DOI：10.1021/la9713311.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Micropatterned arrays of active proteins are vital to the next generation of high-throughput multiplexed biosensors and advanced medical diagnostics. We have developed a simple method for fabricating antibody arrays using a micromolded hydrogel stamper and an aminosilylated receiving surface. The stamping procedure permits direct protein deposition and micropatterning while avoiding cross-contamination of separate patterned regions. Three different antibodies were stamped in adjacent arrays of 50-80 mu m circular areas with retention of activity. I-125 labeling and atomic force microscopy studies showed that the stamper deposited protein as a submonolayer. The fluorescent signal-to-background ratio of labeled bound antigen was greater than 25:1.
Keyword:
ANTIBODIES
SURFACES
IMMOBILIZATION
GOLD
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
3.9
论文数:
5.4W
被引数:
10.6W
机构
暂无机构信息
引用论文
分享收藏
Metabolic activity of the basal ganglia in parkinsonian syndromes in human and non-human primates: A cytochrome oxidase histochemistry study.png)
Neuroscience
NeuroscienceIF0
PREAI
分享收藏
查看更多内容