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Droplet based microfluidics
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DOI：10.1088/0034-4885/75/1/016601.png)
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摘要
En 中文
Droplet based microfluidics is a rapidly growing interdisciplinary field of research combining soft matter physics, biochemistry and microsystems engineering. Its applications range from fast analytical systems or the synthesis of advanced materials to protein crystallization and biological assays for living cells. Precise control of droplet volumes and reliable manipulation of individual droplets such as coalescence, mixing of their contents, and sorting in combination with fast analysis tools allow us to perform chemical reactions inside the droplets under defined conditions. In this paper, we will review available drop generation and manipulation techniques. The main focus of this review is not to be comprehensive and explain all techniques in great detail but to identify and shed light on similarities and underlying physical principles. Since geometry and wetting properties of the microfluidic channels are crucial factors for droplet generation, we also briefly describe typical device fabrication methods in droplet based microfluidics. Examples of applications and reaction schemes which rely on the discussed manipulation techniques are also presented, such as the fabrication of special materials and biophysical experiments.
Keyword:
POLYMERASE-CHAIN-REACTION
CONTINUOUS-FLOW ANALYSIS
SEGMENTED FLOW
SINGLE-CELLS
T-JUNCTION
FEMTOLITER-VOLUME
MONODISPERSE DROPLET
INTERFACIAL-TENSION
EMULSION DROPLETS
CHAOTIC ADVECTION
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20.7
论文数:
1.6K
被引数:
2.4W
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