.png){kind=icon}
返回
Electrophoretic injection within microdevices
.png){kind=icon}
PRE
AI
DOI：10.1021/ac0110045.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
The flexibility of the microfabricated format creates unique opportunities for study of the electrophoretic process. The present work utilizes digital images to capture the motion of DNA samples during pre-electrophoretic processes. A systematic study of DNA loading and strong sample stacking (sample concentration effects) was performed in order to analyze realistic DNA analysis conditions within microdevices. Using digital imaging and microscopy, DNA sample profiles within the injector were analyzed by deconvolving the geometrical intensity profile into different velocity groups. This analysis illustrates the evolution of molecular separation into distinct migrating populations within the injector itself. The present study performed DNA injections within microfabricated devices imposing run voltages between 85 and 850 V/cm. Data from 3 different offset lengths of a double-T cross-injector, 10 different applied voltages, and 2 different sample preparation protocols are presented.
Keyword:
CAPILLARY-ZONE-ELECTROPHORESIS
SAMPLE STACKING
DNA
PRINCIPLES
SEPARATION
REPTATION
GEOMETRY
MATRICES
DEVICES
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
6.7
论文数:
4.7W
被引数:
15.9W
机构
暂无机构信息
引用论文
Characterization of high molecular mass linear polyacrylamide powder prepared by emulsion polymerization as a replaceable polymer matrix for DNA sequencing by capillary electrophoresis.png)
ELECTROPHORESIS
ELECTROPHORESISIF2.5
PREAI
分享收藏Turn geometry for minimizing band broadening in microfabricated capillary electrophoresis channelsANALYTICAL CHEMISTRY
ANALYTICAL CHEMISTRYIF6.7
PREAI
分享收藏分享收藏
查看更多内容