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摘要
En 中文
Demand for DNA sequence information has never been greater, yet current Sanger technology is too costly, time consuming, and labor intensive to meet this ongoing demand. Applications span numerous research interests, including sequence variation studies, comparative genomics and evolution, forensics, and diagnostic and applied therapeutics. Several emerging technologies show promise of delivering next-generation Solutions for fast and affordable genome sequencing. In this review article, the DNA polymerase-dependent strategies of Sanger sequencing, single nucleotide addition, and cyclic reversible termination are discussed to highlight recent advances and potential challenges these technologies face in their development for ultrafast DNA sequencing.
Keyword:
REPLACEABLE LINEAR POLYACRYLAMIDE
CAPILLARY-ARRAY ELECTROPHORESIS
PHOTOCLEAVABLE FLUORESCENT NUCLEOTIDE
OLIGONUCLEOTIDE MICROARRAYS
1000 BASES
POLYMERASE
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PERFORMANCE
GENOMICS
SYSTEM
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IF:
5.5
论文数:
5.6K
被引数:
4.3W
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