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Engineered crRNA for biosensing
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DOI：10.1016/j.tibtech.2024.06.006.png)
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摘要
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基于CRISPR/Cas的诊断方法（CRISPR-Dx）面临挑战，包括检测超短核苷酸的困难、预扩增依赖性、交叉污染、原位检测范式的不足，以及检测非核酸靶标的困难。本论坛概述了在工程化CRISPR RNA（crRNA）方面的进展，以解决上述问题，并着重强调了挑战、机遇和未来方向。
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