.png){kind=icon}
返回
Engineering a Formic Acid Reductase
.png){kind=icon}
PRE
AI
DOI：10.1021/acscatal.5c04079.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
The formate bioeconomy envisions production of formic acid from CO2 via (electro-)chemical conversion, followed by conversion to the product by engineered microbes or cell-free systems. One prominent way of expanding formate valorization is its reduction to formaldehyde, making highly efficient assimilation cascades accessible. This thermodynamically challenging reaction can be catalyzed by ATP-dependent activation followed by NAD(P)H-dependent reduction. Existing solutions rely on two-step cascades, or fusion enzymes thereof, and are limited by the fast hydrolysis of their formylated intermediates. Here, we show that carboxylic acid reductase can be engineered toward formate reduction, resulting in a single-enzyme solution that does not release intermediates. In addition, we discovered that this enzyme tolerates high formate concentrations when used in Escherichia coli whole-cell conversion, conditions that strongly inhibit existing formate reduction cascades. We therefore provide a valuable addition to the toolbox of synthetic formate reduction, providing an enzyme compatible with applications amenable to high formate titers, such as whole-cell bioconversion or electrobiochemical cascades.
Keyword:
formate reduction
enzyme engineering
carboxylicacid reductase
directed evolution
one-carbon metabolism
期刊
IF:
13.1
论文数:
1.6W
被引数:
15.0W
机构
引用论文
分享收藏A new-to-nature photosynthesis system enhances utilization of one-carbon substrates in Escherichia coli一种新近发现的光合系统增强了大肠杆菌对一碳底物的利用.png)
NATURE COMMUNICATIONS
OAAI
NATURE COMMUNICATIONSIF15.7
OAAI分享收藏分享收藏Engineering a new-to-nature cascade for phosphate-dependent formate to formaldehyde conversion in vitro and in vivoNATURE COMMUNICATIONS
OAAI
NATURE COMMUNICATIONSIF15.7
OAAI分享收藏
查看更多内容