.png){kind=icon}
返回
Engineering Programmable DNA Particles and Capsules Using Catechol-Functionalized DNA Block Copolymers
.png){kind=icon}
PRE
AI
DOI：10.1021/acs.chemmater.2c01586.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
DNA block copolymer (DBC) assemblies have attracted attention because of their tunable properties (e.g., programmability, high biocompatibility, efficient cellular uptake, and stability against enzymatic degradation); however, controlling the size of DNA block copolymer assemblies and preparing well-defined DNA-functionalized particle systems are challenging. Herein, we report the preparation of DBC-based particles and capsules with different sizes (i.e., from approximately 0.15 to 3.2 mu m) and a narrow size distribution (i.e., polydispersity index < 0.2) through the assembly of catechol-functionalized DBC, DNA-b-poly(methyl methacrylate-co-2-methacryloylethyl dihydrocaffeate, with metal ions (e.g., FeIII). This assembly process largely exploits the coordination bonding of the metal ions and phenolic (i.e., catechol) groups, forming metal-phenolic networks (MPNs). The DBC-FeIII MPN capsules formed are stable under acidic, metal-chelating, and surfactant solutions because of the coexistence of metal coordination, hydrogen bonding, and hydrophobic interactions. The molecular recognition properties of the DNA strands enable tailorable interactions with small molecules and nanoparticles and are used to tune the permeability of the assembled capsules (> 40% permeability decrease for 2000 kDa fluorescein isothiocyanate dextran compared with untreated capsules). The DBC-FeIII MPN particles show efficient cellular uptake and endosomal escape capability, allowing the efficient delivery of small-interfering RNA for gene silencing (89% downregulation). The reported approach provides the rational design of a range of DNA-functionalized particles, which can potentially be applied in materials science and biomedical applications.
Keyword:
SPHERICAL NUCLEIC-ACIDS
NANOPARTICLES
GROWTH
期刊
IF:
7
论文数:
2.8W
被引数:
11.4W
机构
引用论文
Nanoporous gyroid TiO2 and SnO2 by melt infiltration of block copolymer templates通过嵌段共聚物模板的熔体渗透实现纳米多孔TiO2和SnO2
PREAI
分享收藏A facile method of synthesizing ammonia modified graphene oxide for efficient removal of uranyl ions from aqueous medium.png)
RSC Advances
RSC AdvancesIF0
PREAI
分享收藏
分享收藏
查看更多内容