.png){kind=icon}
返回
Enzyme-Responsive DNA Condensates
.png){kind=icon}
OA
AI
DOI：10.1021/jacs.4c08919.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Membrane-less compartments and organelles are widely acknowledged for their role in regulating cellular processes, and there is an urgent need to harness their full potential as both structural and functional elements of synthetic cells. Despite rapid progress, synthetically recapitulating the nonequilibrium, spatially distributed responses of natural membrane-less organelles remains elusive. Here, we demonstrate that the activity of nucleic-acid cleaving enzymes can be localized within DNA-based membrane-less compartments by sequestering the respective DNA or RNA substrates. Reaction-diffusion processes lead to complex nonequilibrium patterns, dependent on enzyme concentration. By arresting similar dynamic patterns, we spatially organize different substrates in concentric subcompartments, which can be then selectively addressed by different enzymes, demonstrating spatial distribution of enzymatic activity. Besides expanding our ability to engineer advanced biomimetic functions in synthetic membrane-less organelles, our results may facilitate the deployment of DNA-based condensates as microbioreactors or platforms for the detection and quantitation of enzymes and nucleic acids.
Keyword:
LIQUID PHASE-SEPARATION
ARTIFICIAL CELLS
PROTEIN
DIFFUSION
GRANULES
DROPLETS
RNA
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
15.6
论文数:
20.0W
被引数:
60.2W
机构
引用论文
A Synthetic Signaling Network Imitating the Action of Immune Cells in Response to Bacterial Metabolism模拟免疫细胞响应细菌代谢的合成信号网络.png)
ADVANCED MATERIALSOAAI
ADVANCED MATERIALSIF26.8
OAAI分享收藏Co-transcriptional production of programmable RNA condensates and synthetic organelles可编程RNA缩合物和合成细胞器的共转录产生NATURE NANOTECHNOLOGYOAAI
NATURE NANOTECHNOLOGYIF34.9
OAAI分享收藏Engineering DNA-based synthetic condensates with programmable material properties, compositions, and functionalities具有可编程材料特性、组成和功能的基于DNA的工程合成缩合物SCIENCE ADVANCESOAAI
SCIENCE ADVANCESIF12.5
OAAI分享收藏The persistence length of DNA is reached from the persistence length of its null isomer through an internal electrostatic stretching forceBIOPHYSICAL JOURNALOAAI
BIOPHYSICAL JOURNALIF3.1
OAAI分享收藏分享收藏
查看更多内容