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Exploring Ectoine Production From Methanol, Formate, and Electrochemically Produced Formate by Methyloligella halotolerans
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DOI：10.1002/elsc.70063.png)
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Abstract
En 中文
Microbial synthesis using renewable C1-carbon sources like electrochemically produced formate (e-formate) represents a promising approach for climate-neutral chemical production. This study investigates formate utilization for ectoine biosynthesis by the halophilic methylotroph Methyloligella halotolerans. Preliminary growth assays confirmed formate utilization using 15-20 mM formate as the sole energy source substrate, when supplemented with yeast extract or vitamin solutions in a mineral salt medium. In a systematic study for ectoine production, formate utilization reached 0.305 +/- 0.020 mmol d-1 at 20 mM. With different C1-substrates at 20 mM (3 mmol), ectoine production reached 10.3 +/- 3.2 mu mol (from methanol), 6.5 +/- 0.8 mu mol (from equimolar methanol/formate mix), 4.4 +/- 0.1 mu mol (from formate), and 1.2 +/- 0.1 mu mol (from e-formate). Medium buffering, pH stability and toxicity limited performance when formate and e-formate were supplied. Although ectoine yields were suboptimal, the feasibility of e-formate-based ectoine biosynthesis under high-salinity conditions with 9% NaCl, as shown in this study, discloses the great potential for integrating highly efficient electrochemical CO2 reduction in saline media with microbial synthesis of organic chemicals.
Keywords:
C1-substrates
ectoine
electrochemical CO2 reduction
formate utilization
Methyloligella halotolerans
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