Facile green synthesis and characterization of fluorescent nanoprobe based on Ca, K, N-doped carbon quantum dots derived from Aegle marmelos for bioimaging of human neuroblastoma cancer cells and living organisms

Vishal Kansay

doi:10.1016/j.dyepig.2023.111477

Facile green synthesis and characterization of fluorescent nanoprobe based on Ca, K, N-doped carbon quantum dots derived from Aegle marmelos for bioimaging of human neuroblastoma cancer cells and living organisms

2023-10-01

5

PRE

AI

V

Vishal Kansay

V

Varun Dutt Sharma

G

Gourav Chandan

I

Indu Sharma

A

Anita Bhatia

S

Sandip Chakrabarti

M

M. K. Bera *

DOI：10.1016/j.dyepig.2023.111477

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This study demonstrated an environmentally responsible, inexpensive and simple one-step hydrothermal method to synthesize biocompatible fluorescent carbon quantum dots (CQDs) from Aegle marmelos fruit extract. The near -spherical CQDs were discovered to have a naturally distributed size of 3-6 nm, were unintentionally doped with heteroatoms (Ca, K, and N) without the need for doping element precursors due to mineral contents in extract, had a quantum yield of about 4.06%, strong photostability, and excellent water solubility. Several morpholog-ical, compositional, physicochemical, and optical properties were studied. Other variables influencing CQD photoluminescence, such as hydrothermal process parameters and pH sensitivity, were also examined. Zeta potential analysis showed a negative surface charge with a zeta potential value of-5.29 mV and good dis-persibility in aqueous solution. Furthermore, as an alternative to organic or synthetic probes, there is a huge need for the development of simple, affordable, and non-destructive fluorescent biomarkers. As-synthesized Ca, K, N -doped CQDs have demonstrated good fluorescent staining abilities and may be employed as a helpful probe for bio-imaging of cancer cells and other organisms. Cell survival and cytotoxicity were found to be satisfactory, with 90% of SH-SY5Y and HCT-116 cells surviving, compared to 63-77% for the other organisms, even at higher dosages (200 & mu;g/mL) for up to 4 days.

Keywords:

CaKN-doped carbon quantum dots

Green synthesis

Fluorescent biomarker

Bioimaging

Facile green synthesis and characterization of fluorescent nanoprobe based on Ca, K, N-doped carbon quantum dots derived from Aegle marmelos for bioimaging of human neuroblastoma cancer cells and living organisms

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