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Fast data fitting scheme for compressive multispectral fluorescence lifetime imaging
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AI
DOI：10.1364/OL.506378.png)
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摘要
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A single-pixel camera combined with compressive sensing techniques is a promising fluorescence microscope scheme for acquiring a multidimensional dataset (space, spectrum, and lifetime) and for reducing the measurement time with respect to conventional microscope schemes. However, upon completing the acquisition, a computational step is necessary for image reconstruction and data analysis, which can be time-consuming, potentially canceling out the beneficial effect of compressive sensing. In this work, we propose and experimentally validate a fast-fit workflow based on global analysis and multiple linear fits, which significantly reduces the computation time from tens of minutes to less than 1 s. Moreover, as the method is interlaced with the measurement flow, it can be applied in parallel with the acquisitions. (c) 2024 Optica Publishing Group
Keyword:
MICROSCOPY
期刊
IF:
3.3
论文数:
4.0W
被引数:
7.6W
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