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Fast-Structured Illumination Microscopy Based on Dichotomy-Correlation Parameter Estimation (dCOR-SIM)
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DOI：10.1021/acsphotonics.3c01845.png)
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Structured illumination microscopy (SIM) has developed into one of the most significant super-resolution imaging techniques for studying the dynamics of live cells in the life sciences, thanks to the advantage of high photon efficiency. Usually, high-quality SIM super-resolution reconstruction presupposes accurate knowledge of the illumination parameters. However, the conventional iterative cross-correlation (COR) method requires cumbersome and time-consuming computations to realize reliable parameter estimation, posing a great challenge for fast, dynamic super-resolution imaging in complex scenes. In this letter, we propose an efficient and robust SIM algorithm based on dichotomy-correlation parameter estimation (dCOR-SIM), which significantly eliminates the iteration redundancy of conventional COR to enable low-complexity illumination parameter extraction while ensuring precision and noise immunity. Experiments demonstrate that dCOR-SIM can achieve high-accuracy parameter estimation with an efficiency similar to 10 times better than conventional COR for fast, high-quality super-resolution reconstruction in complex experimental environments. We believe that dCOR-SIM, with its limited computational burden and robustness to noise, will facilitate fast, long-term live-cell super-resolution observations.
Keyword:
illumination
SIM
super-resolution
imaging
high-quality
reconstruction
conventional
COR
parameter
fast
complex
estimation
noise
dCOR-SIM
期刊
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6.7
论文数:
5.6K
被引数:
2.5W
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