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FIND-IT: Accelerated trait development for a green evolution
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DOI：10.1126/sciadv.abq2266.png)
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摘要
En 中文
Improved agricultural and industrial production organisms are required to meet the future global food demands and minimize the effects of climate change. A new resource for crop and microbe improvement, designated FIND-IT (Fast Identification of Nucleotide variants by droplet DiglTal PCR), provides ultrafast identification and isolation of predetermined, targeted genetic variants in a screening cycle of less than 10 days. Using large-scale sample pooling in combination with droplet digital PCR (ddPCR) greatly increases the size of low-mutation density and screenable variant libraries and the probability of identifying the variant of interest. The method is validated by screening variant libraries totaling 500,000 barley (Hordeum vulgare) individuals and isolating more than 125 targeted barley gene knockout lines and miRNA or promoter variants enabling functional gene analysis. FIND-IT variants are directly applicable to elite breeding pipelines and minimize time-consuming technical steps to accelerate the evolution of germplasm.
Keyword:
CROP IMPROVEMENT
BARLEY
GENE
REVOLUTION
EXPRESSION
MUTANTS
HORDEUM
WHEAT
MUTATIONS
INDUCTION
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期刊
IF:
12.5
论文数:
2.1W
被引数:
18.1W
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引用论文
Characterization of gibberellin receptor mutants of barley (Hordeum vulgare L.).png)
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MOLECULAR PLANTIF24.1
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